The Red Devils lost the final of the UEFA Europa League 1-0 to Tottenham, with the loss affecting the revenue of the club.
Following the loss, approximately 200 people are expected to lose their jobs in this second wave of redundancies.
Reports from England suggest that staff working with the first team will be among those affected.
Man United in a financial crisis
The sports science, scouting, and medical departments are almost certainly facing cuts. Staff associated with the first team were not given prior notice so they could focus on preparing for the Europa League final, which they lost to Tottenham two days ago.
According to reports from the BBC, the morale around the club continues to plummet and has reached critically low levels.
This comes amidst a remarkably poor season, financial difficulties, and now another round of layoffs.
Earlier this season, the Red Devils already let go of around 250 employees, a move expected to save between £8 and £10 million.
Wednesday's Europa League final defeat will cost the club an estimated £100 million in lost revenue from European competitions next season.